Fig. 5
From: Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity
Analysis of rLbSCD6 and rLbRBP42 binding capacity to the 3′ UTR-II.1-antisense motif. a Secondary structure of the 3′ UTRII.1-antisense motif. Competitive EMSA assays were done using a constant concentration of the rLbSCD6 (b) and rLbRBP42 (c) proteins and a mixture with both 3′ UTR-II.1-dig and cold antisense ribonucleotides. These mixtures contained a constant concentration of 3′ UTR-II.1-dig ribonucleotide and different amounts (1-, 2-, 4- or 8-fold excess) of the antisense ribonucleotide. In lane labeled ‘0 nM’, the reaction mixture contained the 3′ UTR-II.1-dig ribonucleotide, but the antisense ribonucleotide was not present. To confirm the non-interaction between proteins and the antisense motif, an interaction assay was performance using the digoxigenin-labeled antisense ribonucleotide (lane antisense oligo-dig)