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Fig. 5 | Parasites & Vectors

Fig. 5

From: Apoptosis and necroptosis of mouse hippocampal and parenchymal astrocytes, microglia and neurons caused by Angiostrongylus cantonensis infection

Fig. 5

RT-qPCR and western blot analyses of caspase-3, cleaved caspase-3, RIP3 and pRIP3 in parenchymal and hippocampal cells (n = 3). a RT-qPCR products obtained with the total RNA of uninfected mice (0 dpi) and mice at 21 dpi with caspase-3- or RIP3-specific primers. b The expression levels of cleaved caspase-3, RIP3 and pRIP3 in mouse hippocampal and parenchymal cells were confirmed by western blot. c Densitometric analysis of cleaved caspase-3, RIP3 and pRIP3 normalized to the endogenous control (β-actin) and expressed as fold change. The values are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the 0 dpi group

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Parasites & Vectors

ISSN: 1756-3305

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