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Table 1 Analysed sequences of A. phagocytophilum in this herd in combination with clinical symptoms of the infected animals

From: Epidemiology, genetic variants and clinical course of natural infections with Anaplasma phagocytophilum in a dairy cattle herd

Cow no./ tick no./roe deer no.

Date of positive PCR

Ct-value

Clinic

16S rRNA

groEL

msp2

msp4

7

19.06.11

23

not examined

16S-20 (W)

g-18 (X)

M2-26

M4-49

14

04.06.11

17

F, RM

16S-20 (W)

g-18 (X)

M2-26

M4-49

22

30.05.11

15

F, RM, LLO

16S-20 (W)

g-18 (X)

M2-26

M4-49

 

09.07.11

28.5

no clinic

16S-20 (W)

too short

negative

M4-50

 

03.10.11

28

no clinic

16S-20 (W)

g-15 (N)

negative

M4-51

28

03.10.11

20

F, RM, DC

16S-20 (W)

g-15 (N)

negative

M4-51

30

17.05.11

27.5

F, RM, DC

16S-20 (W)

g-18 (X)

too short

M4-50

35

17.05.11

15

F, RM

16S-20 (W)

g-18 (X)

M2-26

M4-49

36

19.05.11

13.5

F, RM, DC

16S-20 (W)

too short

M2-26

M4-49

42

04.09.11

36

not examined

16S-20 (W)

too short

negative

M4-49

46

22.05.11

19.5

F, RM, DC, LLO

16S-20 (W)

g-18 (X)

M2-26

M4-49

 

21.08.11

30

no clinic

negative

negative

negative

M4-50

49

19.05.11

31.5

F, RM, DC, LLO

16S-20 (W)

g-15 (N)

negative

M4-51

52

14.09.11

19.5

F, RM, LLO

16S-20 (W)

too short

M2-27

M4-51

53

22.06.11

14

F, RM, DC

16S-20 (W)

g-18 (X)

negative

M4-49

57

04.06.11

18.5

F, RM, DC

16S-20 (W)

g-18 (X)

M2-26

M4-49

 

04.09.11

30

no clinic

16S-20 (W)

negative

negative

M4-49

58

17.05.11

24.5

F, RM, DC

16S-20 (W)

g-18 (X)

M2-26

M4-49

 

09.07.11

31

no clinic

16S-20 (W)

negative

negative

negative

59

04.09.11

26.5

no clinic

16S-22 (Y)

too short

negative

M4-13 (n)

61

22.06.11

18

F, RM, DC, LLO

16S-20 (W)

g-18 (X)

M2-26

M4-49

 

09.07.11

22

no clinic

16S-20 (W)

negative

M2-27

M4-51

Tick 83a

13.05.11

29

 

16S-7 (I)

negative

negative

negative

Tick 90b

06.06.11

20

 

16S-20 (W)

too short

negative

M4-51

Tick 94b

06.06.11

23

 

16S-20 (W)

too short

negative

M4-49

Tick 96b

06.06.11

19

 

16S-20 (W)

too short

negative

M4-51

Tick 167a

25.06.11

30

 

16S-22 (Y)

too short

negative

M4-13 (n)

Roe deer 1

26.06.11

30

 

16S-21 (X)

negative

M2-9 (J)

too short

Roe deer 2

17.09.11

27.5

 

16S-21 (X)

negative

negative

negative

Roe deer 3

15.10.11

30

 

16S-19 (V)-like sequence

too short

negative

too short

  1. Abbreviations: F fever, RM reduced milk yield, DC discharge from eyes and nose, LLO lower limb oedema and stiff walking
  2. Note: Nomenclature of gene variants is following previous denominations [17, 23, 25] with numbers after the gene abbreviation. The letter codes shown for some of these variants are an alternative, also unofficial nomenclature. Particular combinations of these four gene variants were frequently observed with 16S-20 (W), g-18 (X), M2-26, and M4-49 are given in bold; negative = gene locus could not be amplified; too short = gene locus was amplified but the sequence read was too short for full comparison and thus for allocation to a particular variant. Ct-values are provided as mean of two independent real-time PCRs targeting the msp2-gene, which was used for screening. Please note that tick screening was done only once. A ct-value ≤38 was considered positive for A. phagocytophilum DNA
  3. aCollected from roe deer
  4. bCollected from heifer
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