Fig. 1
Expression profile of oxidative stress genes in BME26 cells in response to microbial stimuli. BME26 cells were infected with either A. marginale or R. rickettsii or exposed to heat-killed M. luteus, E. cloacae, and S. cerevisiae from 6 to 72 h. The gene expression profile of BME26 cells was determined using high-throughput microfluidic RT-qPCR. The relative expression of challenged BME26 cells in relation to control cells was calculated according to the method 2-ΔΔCq. Each cell in the matrix corresponds to the expression level of one gene in a sample (mean values of three biological replicates). The intensity of the colour from green to red indicates the magnitude of differential expression, based on the colour scale at the top of the figure. Abbreviations: (i) Pro-oxidant genes: endoplasmic reticulum oxidoreductin (Rmero1), nitric oxide synthase (Rmnos), dual oxidase-A (Rmduox-A), and dual oxidase-B (Rmduox-B); (ii) Antioxidant genes: manganese superoxide dismutase (RmMnsod), Cu-Zn superoxide dismutase (RmCu/Znsod), catalase (Rmcat), glutathione peroxidase (Rmgpx), phospholipid hydroperoxide glutathione peroxidase (Rmphgpx), glutathione S-transferase (Rmgst1-4), thioredoxin (Rmtrx), thioredoxin reductase (Rmtrxr), and peroxiredoxin (Rmprx)