Fig. 3

Bacterial diversity profiles of the Goanna tick (Bothriocroton undatum). Bacterial abundance and bias at family, order, class and phylum levels were retrieved from a combination of different workflows that included V1-V3 or V3-V4 16S rRNA gene diversity profiles assays, and either Method 1 or Method 2 for the Goanna tick (Bothriocroton undatum). a Graphs with bacterial families and orders show only those with significant bias between the workflows. The graph depicting bacterial classes demonstrates the five most dominant bacterial classes found in each of the workflows, including spirochetes (Borrelia spp.). The graph of bacterial phyla shows no bias found between any of the workflows. Bias between bacterial abundances at different taxonomical levels was evaluated using Tukey’s multiple comparisons test, (P < 0.05) and is indicated with ‘*’. b Venn diagrams representing the number of taxonomic families and genera identified within each 16S rRNA hypervariable region as a measure of richness. A threshold of 30 reads and 97% identification was applied