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Table 2 Mouse bioassay, qPCR on tissue homogenate, and MC-qPCR detection of T. gondii from pooled calf tissues by mouse bioassay (six weeks post-oral inoculation with 106 M4 T. gondii oocysts). Figures in bold indicate a strong positive ELISA or real-time PCR (qPCR) result

From: Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story

Calf pool Tissue Mouse 1 Mouse 2 Tissue homogenate Calf tissues
   Mouse No. Cull date (dpi) ELISA result (%SP) qPCR (Cq) Mouse No. Cull date (dpi) ELISA result (%SP) qPCR (Cq) qPCR (Cq) MC-qPCR (Cq)
Pool 1 = ×2 animals (112, 120) Liver 44-1 42 neg neg 44-3 42 neg neg neg neg
Masseter 44-10 42 neg neg 44-30 42 neg neg neg neg
Tongue 45-1 8 No sample neg 45-3 42 neg neg neg neg
Heart 45-10 42 neg neg 45-30 42 neg neg neg neg
Brain 46-1 6 No sample neg 46-30 3 neg neg neg neg
Diaphragm 46-10 42 neg neg 46-30 42 neg neg neg neg
Left triceps femoralis (forelimb) 47-1 42 neg neg 47-30 42 neg neg 37.20a,c neg
Left semitendinosus (hindlimb) 47-10 42 neg neg 47-30 42 neg neg neg neg
Psoas major (fillet) 48-1 42 neg neg 48-3 42 neg neg neg neg
Longissimus dorsi (sirloin) 48-10 42 neg neg 48-30 42 neg neg neg neg
Pool 1 = ×3 animals (115, 111, 118) Liver 53-1 42 neg 33.79 53-3d 42 neg 33.83a neg 34.40
Masseter 50-10 42 neg neg 50-30 42 neg neg neg 35.54
Tongue 50-1 12 24 19.66 50-3 12 neg 19.04 33.37 32.34
Heart 49-10 42 neg 34.01a 49-30 42 neg 33.53 neg 32.84
Brain 51-1 12 12 21.56 51-3 12 13 21.70 neg neg
Diaphragm 49-1 12 8 23.09 49-3 12 16 21.47 29.04 34.16
Left triceps femoralis (forelimb) 52-1d 42 neg 33.60a 52-3 42 neg neg neg 34.63
Left semitendinosus (hindlimb) 52-10 42 285 b 18.43 52-30 42 230 b 19.87 33.92 32.54
Psoas major (fillet) 53-10 42 neg 18.13 53-30d 42 neg 19.90 neg 34.09
Longissimus dorsi (sirloin) 51-10 42 neg neg 51-30 42 neg neg neg neg
  1. Notes on tissue amounts: 50 g per calf (pool 1, as one calf was removed prior to the end of the experiment) and 33.3 g per calf (pool 2); each pool consisted of 100 g
  2. aA weak positive ELISA or real-time PCR result; ELISA results are expressed as sample to positive control ratios (%S/P); real-time PCR results are recorded as quantification cycle (Cq)
  3. bBetween day 10–14 post-inoculation mice showed signs of T. gondii infection
  4. cRepeated twice and on both occasions one duplicate was negative
  5. dMice positive by total T. gondii tachyzoite immunoblot (52–1, 53–30) or positive in p30 (TgSAG1) immunoblot (52–1, 53–3)
  6. Abbreviation: neg, negative result