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Table 3 Detection of T. gondii by magnetic capture PCR (MC-qPCR). Weight of tissues tested and quantification cycle (Cq) values of DNA extracted from tissues of calves 6 weeks post-oral inoculation with 106 M4 T. gondii oocysts. Figures in bold indicate a positive qPCR result. Calf 109 removed prior to the end of the experiment

From: Detection and dissemination of Toxoplasma gondii in experimentally infected calves, a single test does not tell the whole story

Calf No. Calf pool Tissue
  Heart Liver Diaphragm Triceps femoralis Semitendinosus Masseter
Amount for MC (g) qPCR (Cq) Amount for MC (g) qPCR (Cq) Amount for MC (g) qPCR (Cq) Amount for MC (g) qPCR (Cq) Amount for MC (g) qPCR (Cq) Amount for MC (g) qPCR (Cq)
109 Excl 82 neg 85 neg 74 36.91 91 neg 100 neg 81 neg
112 1 91 37.91 100 neg 8 neg 70 neg 100 neg 95 neg
120 77 neg 95 neg 48 neg 55 neg 57 neg 69 neg
115 2 100 neg 100 neg 56 neg 94 neg 94 neg 50 neg
111 100 neg 100 neg 66 neg 100 neg 100 neg 37 neg
118 89 neg 96 neg na na 65 32.27 98 32.63 69 29.45
  1. Abbreviations: na, insufficient amount of tissue for analysis; neg, negative result
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Parasites & Vectors

ISSN: 1756-3305

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