Fig. 2

Naturally acquired antibody responses to DBL5 domain of VAR2CSA. A selection of sera from malaria-exposed Malawian women was tested for allele-specific or cross-reactive IgG binding to DBL5 in competition ELISA. Panels a-f show examples with cross-reactivity, allele-specificity, or a mixed pattern. Figures show the percent IgG reactivity with increasing concentration of competing DBL5 allele added. Panels a, c, e show results where plates were coated with 7G8 allele and either 7G8 or 3D7 alleles were used as the competitors. Panels b, d, and f show results using the 3D7 allele as the coating antigens. Median values (with IQR) of percentage allele specificity for all individuals (n = 10) tested are also shown in g and h. ** P = 0.0046