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Fig. 3 | Parasites & Vectors

Fig. 3

From: Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding

Fig. 3

SDS-PAGE (a) and Western blotting (b) of recombinant GSTs. a The column farthest left includes markers of molecular weights. Lane 1: bacterial lysate of empty vector; Lane 2: bacterial lysate of HlGST after induction by 1 mM IPTG; Lane 3: bacterial lysate of HlGST after purification by HisTrap affinity chromatography; Lane 4: bacterial lysate of HlGST2 after induction by 1 mM IPTG; Lane 5: bacterial lysate of HlGST2 after purification by HisTrap affinity chromatography. The lysates were run on a 12% SDS-PAGE gel. Gels were stained using Coomassie Blue staining solution. b The column farthest left indicates molecular weight markers. Lane 1: recombinant HlGST; Lane 2: recombinant HlGST2. Bands were visualized using 5-Bromo-4-chloro-3-indolylphosphate/Nitroblue Tetrazolium (BCIP/NBT) Calbiochem® (Merck KGaA, Darmstadt, Germany). Arrowheads indicate the bands for recombinant HlGST and HlGST2 proteins

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Parasites & Vectors

ISSN: 1756-3305

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