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Table 2 Specific activity of each recombinant GST with the substrate CDNB. Values are presented as mean ± SD. The concentration of CDNB and GSH used are 1 mM and 5 mM, respectively. Each experiment was performed at least three times and each assay was run in triplicate

From: Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding

Enzyme

Enzyme activity (units/mg protein)

Equine liver GSTa

11.01 ± 2.60

rHlPRX2b

nd

rHlGSTc

9.24 ± 3.05

rHlGST2d

9.46 ± 1.97

  1. Abbreviation: nd none detected
  2. aEquine liver GST was used as a positive control
  3. bRecombinant H. longicornis peroxiredoxin2 was used as the negative control
  4. cRecombinant HlGST
  5. dRecombinant HlGST2
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Parasites & Vectors

ISSN: 1756-3305

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