Fig. 1

Phylogenetic tree of the genus Midichloria, obtained with Maximum Likelihood analysis of a 16S rRNA gene alignment of long (> 950 nt) sequences. The representative sequence of Midichloria from Hyalomma marginatum obtained in this study (GenBank: LT898326.1) is highlighted in boldface. Bootstrap values above 50 are shown on the respective branches. Scale-bar: 1 nucleotide substitution per 100 positions. Abbreviation: “Ca.”, “Candidatus”