Table 1 Primers and annealing temperature for the identification of G. duodenalis and E. bieuensi
Gene locus | Primer sequences (5’–3’) | Annealing temperature (°C) | Expected product size (bp) | Reference |
|---|---|---|---|---|
bg | F1: AAGCCCGACGACCTCACCCGCAGTGC | 50 | 511 | [19] |
R1: GAGGCCGCCCTGGATCTTCGAGACGAC | ||||
F2: GAACGAACGAGATCGAGGTCCG | 60 | |||
R2: CTCGACGAGCTTCGTGTT | ||||
gdh | F1: TTCCGTRTYCAGTACAACTC | 50 | 530 | [19] |
R1: ACCTCGTTCTGRGTGGCGCA | ||||
F2: ATGACYGAGCTYCAGAGGCACGT | 65 | |||
R2: GTGGCGCARGGCATGATGCA | ||||
tpi | F1: AAATIATGCCTGCTCGTCG | 55 | 530 | [19] |
R1: CAAACCTTITCCGCAAACC | ||||
F2: CCCTTCATCGGIGGTAACTT | 55 | |||
R2: GTGGCCACCACICCCGTGCC | ||||
ITS | F1: GATGGTCATAGGGATGAAGAGCTT | 55 | 392 | [20] |
R1: AATACAGGATCACTTGGATCCGT | ||||
F2: AGGGATGAAGAGCTTCGGCTCTG | 55 | |||
R2: AATATCCCTAATACAGGATCACT | ||||
MS1 | F1: CAAGTTGCAAGTTCAGTGTTTGAA | 58 | 676 | [21] |
R1: GATGAATATGCATCCATTGATGTT | ||||
F2: TTGTAAATCGACCAAATGTGCTAT | 58 | |||
R2: GGACATAAACCACTAATTAATGTAAC | ||||
MS3 | F1: CAAGCACTGTGGTTACTGTT | 55 | 537 | [21] |
R1: AAGTTA GGGCATTTAATAAAATTA | ||||
F2: GTTCAAGTAATTGATACCAGTCT | 55 | |||
R2: CTCATTGAATCTAAATGTGTATAA | ||||
MS4 | F1: GCATATCGTCTCATAGGAACA | 55 | 885 | [21] |
R1: GTTCATGGTTATTAATTCCAGAA | ||||
F2: CGAAGTGTACTACATGTCTCT | 55 | |||
R2: GGACTTTAATAAGTTACCTATAGT | ||||
MS7 | F1: GTTGATCGTCCAGATGGAATT | 55 | 471 | [21] |
R1: GACTATCAGTATTACTGATTATAT | ||||
F2: CAATAGTAAAGGAAGATGGTCA | 55 | |||
R2: CGTCGCTTTGTTTCATAATCTT |