Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau

Table 2 Primers for amplification of seven overlapping DNA fragments and their positions in the mtDNA of Echinococcus (G10) isolate from Gansu, China, and four additional primer pairs used to amplify a region containing SNR. The positions of the primers are based on the mt genome sequence of E. canadensis (G10; AB745463)

Primer name	Primer sequence (5′ → 3′)	Positions on the H-strand	Size of PCR product (bp)
F1	TTTGTAAAGATGCCAGAAAA	244–266	2110
R1	AYCTAGATCATTTTTTTGGA	2337–2356	2110
F2	GCCCCATATATGTATAGTAT	2225–2244	1962
R2	TATACACCGAAGAATAGCAT	3897–3916	1962
F3	GATTTRGTGTATTTTCATTCRTA	3710–3732	2508
R3	CCAAAACACCCTAACCTAATAT	6196–6217	2508
F4	ATCGTTTGCCWTATTGTTATAG	5965–5986	2622
R4	TAACGGAAAATAAATTCACA	8567–8586	2622
F5	TGCTGTTAACTTCAAGAAATGG	8418–8439	1885
R5	ACATAACATAATGAAAATGAGC	10,281–10,302	1885
F6	ATATGTTTACTGTTGGGTTRGAT	10,027–10,049	2352
R6	GCAGCACATAGACTTGGCTT	12,360–12,379	2352
F7	CATCTGCGGTTARTCTGTTTTC	12,036–12,057	2143
R7	TAATGCTTAAAACTAACTCATA	437–458	2143
F8	TTTATTTTTGTGTCGGTGTTTG	13,448–13,469	1387
R8	CCCGCATAGCCTCCAACAA	1096–1114	1387
F9	TTCTGGTGTTAAGTGTTGTG	13,659–13,678	498
R9	TAACTTCTGACATAGCTACC	417–436	498
F10	GGCTTGTGTGTATTATTTGG	13,514–13,533	793
R10	ACAAACCTATACTAACACAC	567–586	793
F11	GGTGTTAAGTGTTGTGGCCAGAAA	13,663–13,686	530
R11	GAAACATCCATAATTAATGCTTAAAACTAACTC	440–472	530

ISSN: 1756-3305