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Fig. 6 | Parasites & Vectors

Fig. 6

From: The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells

Fig. 6

Ehtrf1 is downregulated in response to UV irradiation and heat-shock stress. UV-irradiation a-e Relative gene expression of Ehtbp (a), Ehtrf1 (b), Ehrad54 (c), Ehblm (d) and Ehpcna (e) normalized with the endogenous gene control Eh40Ss2. f Gene expression analysis of Ehtbp and Ehtrf1 by RT-qPCR in trophozoites cultured at 37 °C and 42 °C as described. g As a control of heat-shock response, the expression level of Ehhsp70 gene was determined. No change in morphology was observed in both cases. Significant values are marked with asterisks (Student’s t-test, *P < 0.05, **P < 0.001, ***P < 0.0005)

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