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Fig. 7 | Parasites & Vectors

Fig. 7

From: The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells

Fig. 7

Gene expression analyses of Ehtrf1 and Ehtbp genes of E. histolytica trophozoites during erythrophagocytosis and MDCK monolayer destruction and amoebic abscess formation. a-d Erythrophagocytosis assay. a Representative photomicrographs showing the accumulation of ingested erythrocytes in trophozoites at different time points. Photomicrographs were taken at 200× magnification. b Graph showing the average number of ingested erythrocytes by trophozoites. c, d Gene expression levels of Ehtbp (c) and Ehtrf1 (d). e-g MDCK cell monolayer destruction by trophozoites. e Images of representative culture plates of stained MDCK cell monolayers destroyed by live trophozoites interacting for 5, 15 and 30 min. An intact MDCK cell monolayer is shown (0 min) for comparison. f, g RT-qPCR analysis of Ehtbp and Ehtrf1 genes, respectively. Statistical analysis with significant difference are marked with asterisks (Student’s t-test, *P < 0.05, **P < 0.001, ***P < 0.0003). Scale-bar: 10 μm

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