Table 1 Nucleotide sequences of primers and TaqMan MGB probes used in the duplex qPCR assay
From: Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis
Oligonucleotide name | Nucleotide sequence (5'-3') and modifications | Reference |
|---|---|---|
TeEMA1-F | CTGACTACAAGGTYGTATAC | This study |
TeEMA1-R | TGTCGTCACTTAGTAAAATAGA | This study |
TeEMA1-P | 6-FAM-TTCTCCGTCTATGGCGCA-MGB-NFQ | This study |
Bc_18SF402 | GTAATTGGAATGATGGCGACTTAA | Bhoora et al. [27] |
Bc_18SR496 | CGCTATTGGAGCTGGAATTACC | Bhoora et al. [27] |
Bc_18SP | VIC-CCTCGCCAGAGTAA-MGB-NFQ | Bhoora et al. [27] |