Fig. 8

Histopathological analysis of liver and intestine and levels of enzyme indicators. a-f Liver slides by using Masson’s trichrome staining. Collagen fibers were dyed blue. a-c Representative fields of liver tissues from mice intraperitoneally injected with PBS, B.s-CotC spores, B.s-CotC-CsPmy spores. d-f Representative fields of liver tissues from mice intragastrically immunized with PBS, B.s-CotC spores and B.s-CotC-CsPmy spores, respectively. The yellow arrows indicate adenomatoid hyperplasia, the black arrows indicate the collagen deposition around the bile duct. g Statistical analysis of Ishak scores in liver sections from each group (**P < 0.01, ***P < 0.001). h-m Histological detection of the intestines from orally immunized mice by H&E staining: h, k PBS group; i, l B.s-CotC group; j, m B.s-CotC-CsPmy group (h-j, 100×; k-m, 200×). n The activities of GPT and GOT in sera of intragastrically immunized mice. Scale-bars: a-f, 50 μm; h-m, 100 μm