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Table 1 Occurrence and distribution of Enterocytozoon bieneusi ITS genotypes and multilocus genotypes (MLGs) in pre-weaned dairy calves on five farms in Shanghai, China

From: Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves

Farm

Farm ranka

Sampling point

Sample size

No. positive for E. bieneusi (%)

ITS genotype (No.)

MLG (No.)

1

D

1

36

12 (33.3)

BEB4 (11), Mixed infection (1)

MLG-B1 (2)

2

12

3 (25.0)

BEB4 (3)

3

46

13 (28.3)

BEB4 (13)

MLG-B1 (2), MLG-B2 (1)

4

25

5 (20.0)

BEB4 (5)

MLG-B2 (1)

Subtotal

  

119

33 (27.7)

BEB4 (32), Mixed infection (1)

MLG-B1 (4), MLG-B2 (2)

2

A

1

47

6 (12.8)

CHN4 (4), Type IV and BEB4 (2)

2

9

3 (33.3)

BEB4 (1), Type IV and BEB4 (2)

MLG-B1 (1)

Subtotal

  

56

9 (16.1)

CHN4 (4), BEB4 (1), Type IV and BEB4 (4)

MLG-B1 (1)

3

B

1

112

44 (39.3)

J (42), BEB4 (1), CHN15 (1)

MLG-J1 (1), MLG-J2 (1), MLG-J4 (1)

2

43

17 (39.5)

J (17)

MLG-J3 (1), MLG-J5 (1)

3

81

24 (29.6)

J (24)

MLG-J2 (4)

4

84

19 (22.6)

J (19)

MLG-J2 (1)

5

69

27 (39.1)

J (27)

MLG-J2 (4)

Subtotal

  

389

131 (33.7)

J (129), BEB4 (1), CHN15 (1)

MLG-J2 (10), MLG-J1 (1), MLG-J3 (1), MLG-J4 (1), MLG-J5 (1)

4

E

1

29

12 (41.4)

J (12)

MLG-J6 (3), MLG-J7 (1), MLG-J8 (1)

2

10

3 (30.0)

J (3)

MLG-J6 (1)

Subtotal

  

39

15 (38.5)

J (15)

MLG-J6 (4), MLG-J7 (1), MLG-J8 (1)

5

C

1

109

21 (19.3)

BEB4 (20), J (1)

MLG-B1 (7), MLG-B2 (1)

2

97

5 (5.2)

BEB4 (5)

Subtotal

  

206

26 (12.6)

BEB4 (25), J (1)

MLG-B1 (7), MLG-B2 (1)

Total

  

809

214 (26.5)

J (145), BEB4 (59), CHN4 (4), Type IV and BEB4 (4), CHN15 (1), Mixed infection (1)

MLG-B1 (12), MLG-J2 (10), MLG-J6 (4), MLG-B2 (3), MLG-J1 (1), MLG-J3 (1), MLG-J4 (1), MLG-J5 (1), MLG-J7 (1), MLG-J8 (1)

  1. aFarm ranks A-E were ranking scores used to evaluate the hygiene status, animal density and facility condition, with A representing “excellent” and E representing “very poor” (see [35] for details)