Endemic, exotic and novel apicomplexan parasites detected during a national study of ticks from companion animals in Australia

Table 2 Summary of primer properties

Target gene	Target organisms	Primer name	Primer sequence (5'-3')	Expected amplicon length (bp)	Annealing temperature (°C)	Reference
18S	Piroplasms and Hepatozoon spp.	18SApiF	ACGAACGAGACCTTAACCTGCTA	300	58	This study
	Piroplasms and Hepatozoon spp.	18SApiR	GGATCACTCGATCGGTAGGAG	300	58	This study
	Babesia spp. and Theileria spp.	BTF1 (external)	GGCTCATTACAACAGTTATAG	930	58	[11]
		BTR1 (external)	CCCAAAGACTTTGATTTCTCTC	930	58
		BTF2 (internal)	CCGTGCTAATTGTAGGGCTAATAC	800	62
		BTR2 (internal)	GGACTACGACGGTATCTGATCG	800	62
		Nbab_1F	AAGCCATGCATGTCTAAGTATAAGCTTTT	1500	60	[13]
		18SApiR	GGATCACTCGATCGGTAGGAG	1500	60	This study
	Hepatozoon spp.	HepF300	GTTTCTGACCTATCAGCTTTCGACG	600	60	[16]
		Hep900	CAAATCTAAGAATTTCACCTCTGAC	600	60	[16]
		HEMO1	TATTGGTTTTAAGAACTAATTTTATGATTG	900	48	[17]
		HEMO2	CTTCTCCTTCCTTTAAGTGATAAGGTTCAC	900	48	[17]
		HAM-1F	GCCAGTAGTCATATGCTTGTC	1650	56	[18]
		HPF-2R	GACTTCTCCTTCGTCTAAG	1650	56	[18]
MPSP p32	Theileria orientalis	Ts-U	CACGCTATGTTGTCCAAGAG	800	58C	[15, 19]^a
MPSP p32	Theileria orientalis	Ts-R	TGTGAGACTCAATGCGCCTA	800	58C	[15, 19]^a

^aPrimers were designed by Tanaka et al. [19] and PCR assay conditions were followed according to Zakimi et al. [15]

ISSN: 1756-3305