Fig. 1

Sampling sites and diversity of wPip in Cx. pipiens (s.l.) populations in Turkey. A total of 753 samples were collected from 59 different sampling sites, tested with a PCR/RFLP assay on the pk1 gene (1.3 kb) and assigned to one of the five genetically distinct wPip groups (wPip-I to V). Results showed the occurrence of wPip from three different groups in the area namely wPip-I-II and IV. Size of the circle represents the sampling size. Percentage of a given wPip group in a given population is shown in different colours; blue: wPip-I, green: wPip-II, pink: wPip-IV as defined in Dumas et al. [16]. Star indicates the location of the samples (Tekirdag, sampling site 52) that have been used to establish Tek wPip-I and Tek wPip-II lines. Reciprocal crosses between these lines and additional reference lines were performed to identify natural CI patterns caused by wPip groups in this region