Anaplasma phagocytophilum, Bartonella spp., haemoplasma species and Hepatozoon spp. in ticks infesting cats: a large-scale survey

Table 1 Details of the qPCR/PCR assays used in the study for the detection of tick-borne pathogens

Target species (target gene)	PCR primer and probe sequences (5'–3')	Product size (bp)	Reference
Anaplasma phagocytophilum (msp2)	F: ATGGAAGGTAGTGTTGGTTATGGTATT	77	[7]
	R: TTGGTCTTGAAGCGCTCGTA
	FAM-TGGTGCCAGGGTTGAGCTTGAGATTG-BHQ1
Bartonella henselae (alr-gcvP intergenic spacer)	F: GAGGGAAATGACTCTCTCAGTAAAA	110	[9]^a
	R: TGAACAGGATGTGGAAGAAGG
	FAM-CAGCCAAATATACGGGCTATCCATCAA-BHQ1
Bartonella spp. (ssrA)	F: GCTATGGTAATAAATGGACAATGAAATAA	299	[8]^b
	R: GGCTTCTGTTGCCAGGTG
	FAM-ACCCCGCTTAAACCTGCGACG-BHQ1
“Candidatus Mycoplasma haemominutum” (16S rRNA gene)	F: TGATCTATTGTKAAAGGCACTTGCT	135	[10]
	R: TTAGCCTCYGGTGTTCCTCAA
	FAM-TTCAATGTGTAGCGGTGGAATGCGT-BHQ1
“Candidatus Mycoplasma turicensis” (16S rRNA gene)	F: AGAGGCGAAGGCGAAAACT	138	[10]
	R: ACGTAAGCTACAACGCCGAAA
	FAM-CGTAAACGATGGGTATTAGATGTCGGGAT-BHQ1
Feline genomic DNA (28S rRNA)	F: AGCAGGAGGTGTTGGAAGAG	100	[10]
	R: AGGGAGAGCCTAAATCAAAGG
	Texas Red-TGG CTT GTG GCA GCC AAG TGT-BHQ2
Hepatozoon. spp. (18S rRNA gene)	F: AAACGGCTACCACATNTAAGGA	522	[11]
Hepatozoon. spp. (18S rRNA gene)	R: AATACAAATGCCCCCAACTNT	522	[11]
Mycoplasma haemofelis (16S rRNA gene)	F: GTGCTACAATGGCGAACACA	80	[10]
	R: TCCTATCCGAACTGAGACGAA
	FAM-TGTGTTGCAAACCAGCGATGGT-BHQ1

^aThe reverse and probe sequences in the original paper are incorrectly labelled; the correct sequences are cited in this table
^bThe reverse primer has been modified compared to the one described in the paper
Abbreviations: F, forward primer sequence; R, reverse primer sequence; FAM, 6-carboxyfluorescein; BHQ, black hole quencher (1 or 2 as indicated)

ISSN: 1756-3305