A novel Babesia orientalis 135-kilodalton spherical body protein like: identification of its secretion into cytoplasm of infected erythrocytes

Table 1 Primers used for the subcloning and sequencing of recombinant plasmids

Primer	Primer sequence (5'-3')	Restriction endonuclease
SBP3-like-F	ATGAGGCGCTTCACCCTGCTAGCGTTG
SBP3-like-R	TTACATGTCCATACCGACGCGGCATAG
SBP3-like-F1	CGGATCCATGAGGCGCTTCACCCTG	BamHI
SBP3-like-R1	GCGTCGACAATCTGAGTGAAGTTGACG	SalI
SBP3-like-F2	GGAATTCATGGCCCCACCTTCTGCC	EcoRI
SBP3-like-R2	GCGTCGACTTACTGGAGGAAGAACTGG	SalI
SBP3-like-F3	CGGATCCATGATCACTGCTTACGAC	EcoRI
SBP3-like-R3	GCGTCGACTTACATGTCCATACCGACG	Sal I

ISSN: 1756-3305