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Table 3 PCR and typing results of the semi-nested PCR at the SSU RNA gene locus (SSU PCR)

From: Occurrence and distribution of Giardia species in wild rodents in Germany

Animals Typed Giardia microti Giardia muris Giardia duodenalis d Octomitus intestinalis Non-typeable Neg. (PCR)
Genus Species No. of positive samples (n) n (%) 95% CI (%) n (%) 95% CI (%) n (%) 95% CI (%) n (%) 95% CI (%) n n
Apodemus agrarius 24 (35) 2   21       1 11
  flavicollis 13 (38) 5   8        25
  sylvaticus 2 (9)    2        7
Totale   39 (93) 7 (17.9)a, b 9.3–36.5 31 (79.5)a, b 60.7–88.9 1 (2.6) 0.6–13.5    1 53
Microtus agrestis 52 (60) 49   2     1    8
  arvalis 81 (107) 78       1   2 26
Totalf   138 (175) 134 (97.1)a, c 92.7–99.2 2 (1.4) a, c 0.2–5.1    2 (1.4) 0.2–5.1 2 35
Myodes glareolus 181 (301) 173 (95.6)b, c 91.5–98.1 3 (1.7)b, c 0.3–4.8 4 (2.2) 0.6–5.6 1 (0.6) 0.01–3.0 10 110
Total   358 (571) 314 (87.7) 83.9–90.9 36 (9.8) 6.9–13.3 5 (1.4) 0.5–3.2 3 (0.8) 0.2–2.4 13 200
  1. Abbreviations: n sample size, CI confidence interval, neg negative
  2. a-cTest for any difference of proportions (G. microti, G. muris) from rodent category (Apodemus, Microtus, Myodes) was done using Fisher-Freeman-Halton test, P-value < 0.0001. Comparison between groups was done by using Fisher’s exact test followed by multiple testing correction (Bonferroni-Holm procedure): aApodemus vs Microtus, P-value (adjusted) < 0.0001. bApodemus vs Myodes, P-value (adjusted) < 0.0001. cMicrotus vs Myodes, P-value (adjusted) = 1
  3. dG. duodenalis assemblage A was found in 3 animals (1 Apodemus, 2 Myodes), G. duodenalis assemblage B was found in 2 animals (both Myodes)
  4. eIncluding 6 animals for which A. flavicollis and A. sylvaticus could not be discriminated
  5. fIncluding 8 animals for which Microtus spp. could not be discriminated