Occurrence and distribution of Giardia species in wild rodents in Germany

Parasites & Vectors

Table 3 PCR and typing results of the semi-nested PCR at the SSU RNA gene locus (SSU PCR)

Animals		Typed	Giardia microti		Giardia muris		Giardia duodenalis ^d		Octomitus intestinalis		Non-typeable	Neg. (PCR)
Genus	Species	No. of positive samples (n)	n (%)	95% CI (%)	n (%)	95% CI (%)	n (%)	95% CI (%)	n (%)	95% CI (%)	n	n
Apodemus	agrarius	24 (35)	2		21						1	11
	flavicollis	13 (38)	5		8							25
	sylvaticus	2 (9)			2							7
Total^e		39 (93)	7 (17.9)^{a, b}	9.3–36.5	31 (79.5)^{a, b}	60.7–88.9	1 (2.6)	0.6–13.5			1	53
Microtus	agrestis	52 (60)	49		2				1			8
	arvalis	81 (107)	78						1		2	26
Total^f		138 (175)	134 (97.1)^{a, c}	92.7–99.2	2 (1.4) ^{a, c}	0.2–5.1			2 (1.4)	0.2–5.1	2	35
Myodes	glareolus	181 (301)	173 (95.6)^{b, c}	91.5–98.1	3 (1.7)^{b, c}	0.3–4.8	4 (2.2)	0.6–5.6	1 (0.6)	0.01–3.0	10	110
Total		358 (571)	314 (87.7)	83.9–90.9	36 (9.8)	6.9–13.3	5 (1.4)	0.5–3.2	3 (0.8)	0.2–2.4	13	200

Abbreviations: n sample size, CI confidence interval, neg negative
^a-cTest for any difference of proportions (G. microti, G. muris) from rodent category (Apodemus, Microtus, Myodes) was done using Fisher-Freeman-Halton test, P-value < 0.0001. Comparison between groups was done by using Fisher’s exact test followed by multiple testing correction (Bonferroni-Holm procedure): ^aApodemus vs Microtus, P-value (adjusted) < 0.0001. ^bApodemus vs Myodes, P-value (adjusted) < 0.0001. ^cMicrotus vs Myodes, P-value (adjusted) = 1
^dG. duodenalis assemblage A was found in 3 animals (1 Apodemus, 2 Myodes), G. duodenalis assemblage B was found in 2 animals (both Myodes)
^eIncluding 6 animals for which A. flavicollis and A. sylvaticus could not be discriminated
^fIncluding 8 animals for which Microtus spp. could not be discriminated

ISSN: 1756-3305