Table 1 Primers for PCR amplification and qPCR reaction
From: Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks
Organism | Target gene | Primer | Sequence (5'-3') | Reference |
|---|---|---|---|---|
Bacteria | 16S | 338f | ACTCCTRCGGGAGGCAGCAG | [19] |
806r | GGACTACCVGGGTATCTAAT | |||
R. haemaphysaloides | Actin gene | Ractin-F | GTGCCCATCTACGAAGGTTAC | This study |
Ractin-R | CCATCTCCTGCTCGAAGTCC | |||
Rickettsia-like endosymbiont | Citrate synthase gene (gltA) | gltA-F | TCCTACATGCCGACCATGAG | [20] |
gltA-R | AAAGGGTTAGCTCCGGATGAG | |||
Coxiella-like endosymbiont | 16S rRNA gene | L-CoxF | TGAGTGTTGACGTTACCCACAG | This study |
L-CoxR | GCATTTCACCGCTACACCG |