Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids

Table 1 The oligonucleotides used in this study

Primer name	Primer sequence (5'–3')	Genome position	Primer length (bp)	GenBank ID	Method	Reference
PKD-RPA_F	TAATACGGATGTGGGTTAGTGGAAACTGGG	48–77	30	JQ424926	RPA	This study
PKD-RPA_R	Digoxigenin-CGTGAATCAGACCAAATATCTTCAAGATGACAAG	155–188	34
PKD-RPA_P	FAM-GGAGTGGGAAGATAATACGTAAACGTGCATTZGCCAAGTATTTAATAG-(C3spacer)	106–153	48
PKX 5 F	CCTATTCAATTGAGTAGGAGA	463–483	21	U70623	PCR	[36]
PKX 6 R	GGACCTTACTCGTTTCCGACC	877–897	21	U70623	PCR	[36]

ISSN: 1756-3305