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Table 1 Results obtained by using DNA isolated with QIAgen columns with the different instruments in Experiment 1

From: Evaluation of fluorimetry and direct visualization to interpret results of a loop-mediated isothermal amplification kit to detect Leishmania DNA

Instrument L. infantum 10-fold DNA dilutions of parasite equivalents/μl
  103 102 10 1 10-1 10-2 10-3 10-4 10-5 10-6 10-7
LF-160 Pos Pos Pos Pos Pos Pos Pos Neg Neg Neg Neg
Genie III® Pos Pos Pos Pos Pos Pos Pos Neg Neg Neg Neg
Tp (min:sec) 13:30 14:30 16:15 17:45 21:45 24:00 34:15 0 0 0 0
ESEQuant TS2.2 Pos Pos Pos Pos Pos Pos Pos Neg Neg Neg Neg
LnPCR Pos Pos Pos Pos Pos Pos Neg Neg Neg Neg Neg
qPCR Pos Pos Pos Pos Pos Pos Neg Neg Neg Neg Neg
Ct 19.1 22.5 25.6 28.4 30.7 32.1 Neg Neg Neg Neg Neg
  1. Abbreviations: Pos positive, Neg negative