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Table 2 Summary statistics on the effect of the addition of monkey kidney cells in various serum/protein supplemented basic media with regards to the mean values of T90 of L. loa L3 and microfilariae

From: Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa

Medium Serum/ Protein Concentration (%) Loa loa L3 Loa loa mf
Mann-Whitney U P-value Mann-Whitney U P-value
DMEM No serum   32 0.0084 24 0.0139
Albumax 0.5 16 0.0452 12 0.0471
1 16 0.0476 35 0.0025
1.5 21 0.0467 16 0.0286
BSA 0.5 15 0.0167 12 0.0471
1 19 0.0171 48 0.0006
1.5 14 0.0462 32 0.0040
FBS 5 6 0.5462 15 0.0357
10 15 0.0167 24 0.0095
15 12 0.0448 35 0.0025
NCS 5 15 0.0167 6 0.0200
10 22 0.0381 20 0.0159
15 24 0.0095 20 0.0158
IMDM No serum   25 0.4945 56 0.0003
Albumax 0.5 20.5 0.7479 54 0.0004
1 36 0.0137 80 0.0004
1.5 21.5 0.9999 70 0.0001
BSA 0.5 14.5 0.6303 36 0.0069
1 32 0.0345 96 0.0002
1.5 25 0.9497 96 0.0002
FBS 5 26 0.024 18 0.0257
10 12 0.1419 91 0.0003
15 11 0.1079 72 0.0490
NCS 5 31 0.0411 54 0.0004
10 38 0.0813 96 < 0.0001
15 31 0.0414 96 < 0.0001
RPMI No serum   26 0.0109 98 0.0003
Albumax 0.5 14 0.2413 32.5 0.3170
1 20 0.1087 83 0.0685
1.5 10 0.7483 86 0.1764
BSA 0.5 6 0.9999 40 0.002
1 9 0.4000 77 < 0.0001
1.5 17 0.0111 77 0.0006
FBS 5 15 0.3571 35 0.0057
10 16 0.2857 56 < 0.0001
15 16 0.1905 91 0.0004
NCS 5 6.5 0.9999 29 0.0282
10 9 0.4000 88 0.0003
15 13 0.2000 88 0.0003