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Table 2 Summary statistics on the effect of the addition of monkey kidney cells in various serum/protein supplemented basic media with regards to the mean values of T90 of L. loa L3 and microfilariae

From: Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa

Medium

Serum/ Protein

Concentration (%)

Loa loa L3

Loa loa mf

Mann-Whitney U

P-value

Mann-Whitney U

P-value

DMEM

No serum

 

32

0.0084

24

0.0139

Albumax

0.5

16

0.0452

12

0.0471

1

16

0.0476

35

0.0025

1.5

21

0.0467

16

0.0286

BSA

0.5

15

0.0167

12

0.0471

1

19

0.0171

48

0.0006

1.5

14

0.0462

32

0.0040

FBS

5

6

0.5462

15

0.0357

10

15

0.0167

24

0.0095

15

12

0.0448

35

0.0025

NCS

5

15

0.0167

6

0.0200

10

22

0.0381

20

0.0159

15

24

0.0095

20

0.0158

IMDM

No serum

 

25

0.4945

56

0.0003

Albumax

0.5

20.5

0.7479

54

0.0004

1

36

0.0137

80

0.0004

1.5

21.5

0.9999

70

0.0001

BSA

0.5

14.5

0.6303

36

0.0069

1

32

0.0345

96

0.0002

1.5

25

0.9497

96

0.0002

FBS

5

26

0.024

18

0.0257

10

12

0.1419

91

0.0003

15

11

0.1079

72

0.0490

NCS

5

31

0.0411

54

0.0004

10

38

0.0813

96

< 0.0001

15

31

0.0414

96

< 0.0001

RPMI

No serum

 

26

0.0109

98

0.0003

Albumax

0.5

14

0.2413

32.5

0.3170

1

20

0.1087

83

0.0685

1.5

10

0.7483

86

0.1764

BSA

0.5

6

0.9999

40

0.002

1

9

0.4000

77

< 0.0001

1.5

17

0.0111

77

0.0006

FBS

5

15

0.3571

35

0.0057

10

16

0.2857

56

< 0.0001

15

16

0.1905

91

0.0004

NCS

5

6.5

0.9999

29

0.0282

10

9

0.4000

88

0.0003

15

13

0.2000

88

0.0003