Table 4 Cut-off values, sensitivity, specificity, and positive and negative predictive values of the P. heterotremus 35 kDa recombinant fusion protein ELISA. The ELISA was performed using 1 μg of P. heterotremus 35 kDa recombinant fusion protein and 30 negative serum samples diluted at 1:2000. Test results were determined by the OD405 values of all serum samples under the cut-off value of 0.54
Cut-off value | OD at 405 nm | Sensitivity (%) | Specificity (%) | Predictive values (%) | |
|---|---|---|---|---|---|
Positive | Negative | ||||
Mean + SD | 0.44 | 80 | 80 | 80 | 80 |
Mean + 2 SD | 0.54 | 100 | 100 | 100 | 100 |
Mean + 3 SD | 0.64 | 0 | 100 | 0 | 50 |
Evaluation of test under the cut-off value in this study | |||||
Mean + 2 SD | 0.54 | 88.89 | 95.51 | 74.42 | 98.32 |