Table 1 PCR primers used for Babesia spp., Rickettsia spp. and Anaplasma spp. and C. burnetii screening
Target agent | PCR method | Primer | Primer sequences (5'→3') | Target gene | Amplicon size (bp) | Tissue tested | Reference |
|---|---|---|---|---|---|---|---|
Babesia spp. | PCR | BJ1 | GTCTTGTAATTGGAATGATGG | 18S rDNA | ~500 | Blood | [10] |
BN2 | TAGTTTATGGTTAGGACTACG | ||||||
Nested PCR | Bab_For1 | ATWGGATTYTATATGAGTAT | cox1 | 924 | [7] | ||
Bab_Rev1 | ATAATCWGGWATYCTCCTTGG | ||||||
Bab_For2 | TCTCTWCATGGWTTAATTATGATAT | ||||||
Bab_Rev2 | TAGCTCCAATTGAHARWACAAAGTG | ||||||
Rickettsia spp. | qPCR | gltA-F | GTGAATGAAAGATTACACTATTTAT | gltA | – | Blood | [30] |
gltA-R | GTATCTTAGCAATCATTCTAATAGC | ||||||
qPCR | 338-F | GAMAAATGAATTATATACGCCGCAAA | RC0338 gene | – | |||
338-R | ATTATTKCCAAATATTCGTCCTGTAC | ||||||
Anaplasma spp. | Nested PCR | AE1-F | AAGCTTAACACATGCAAGTCGAA | 16S rRNA | 926 | Blood | [31] |
AE1-R | AGTCACTGACCCAACCTTAAATG | ||||||
EE3 | GTCGAACGGATTATTCTTTATAGCTTGC | ||||||
EE4 | CCCTTCCGTTAAGAAGGATCTAATCTCC | ||||||
Coxiella burnetii | Nested PCR | omp1 | AGTAGAAGCATCCCAAGCATTG | com1 | 438 | Spleen | [32] |
omp2 | TGCCTGCTAGCTGTAACGATTG | ||||||
omp3 | GAAGCGCAACAAGAAGAACA | ||||||
omp4 | TGGAAGTTATCACGCAGTTG |