Differential modulation of host immune genes in the kidney and cranium of the rainbow trout ( Oncorhynchus mykiss ) in response to Tetracapsuloides bryosalmonae and Myxobolus cerebralis co-infections

Parasites & Vectors

Table 1 List of quantitative real-time PCR primers

Primer name	Primer sequence (5′-3′)	Amplicon size (bp)	GenBank ID	Reference
T. bryosalmonae RPL18 F	GTAAACGGGGACAAAAAGA	251	FR852769	[8]
T. bryosalmonae RPL18 R	GGAGCAGCACCAAAATAC	251	FR852769	[8]
Myx18-909 F	CTTTGACTGAATGTTATTCAGTTACAGCA	88	AF115253	[39]
Myx18-996 R	GCGGTCTGGGCAAATGC	88	AF115253	[39]
SOCS-1 F	GATTAATACCGCTGGGATTCTGTG	136	AM748721	[8]
SOCS-1 R	CTCTCCCATCGCTACACAGTTCC	136	AM748721	[8]
SOCS-3 F	CACAGAGAAACCGTTAAAAGGACTATCC	228	AM748723	[8]
SOCS-3 R	AAGGGGCTGCTGCTCATGAC	228	AM748723	[8]
JAK-1 F	ACACTGATATTGGGCCGTTCTGGA	174	CA378782	[40]
JAK-1 R	CCTCGTCCTCTGCATCTTTACCAAC	174	CA378782	[40]
STAT-3 F	GAATGAAGGGTATATTCTGG	152	U60333	[41]
STAT-3 R	TCCCACTGATGTCCTTTTCC	152	U60333	[41]
EF-1α F	AGACAGCAAAAACGACCCCC	167	HF563594	[42]
EF-1α R	AACGACGGTCGATCTTCTCC	167	HF563594	[42]

These primers were used in RT-qPCR to quantify the relative gene expression in posterior kidneys and cranial cartilages of infected rainbow trout during single and co-infections