Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones

Table 2 Experimental infection of sheep for production of Fasciola hepatica clonal isolates

Clonal isolate	Total no. of cercariae shed by a single snail used to derive clonal infections	No. of metacercariae used for infection	Timepoint of PM (wpi)	No. of adult flukes recovered at PM^a
FhLivR1	931	150	18	70
FhLivR1	931	220	18	165 (T)
FhLivR2	553	193	18	31
FhLivR2	553	200	18	48 (T)
FhLivR3	812	256	18	35 (T)
FhLivS1	3200	209	19	15
FhLivS1	3200	224	19	0 (T)
FhLivS2	> 417^b	215	24	113
FhLivS2	> 417^b	202	24	0 (T)
FhLivS3	1166	200	17	127
FhLivS3	1166	200	17	0 (T)

^aTwo sheep were infected for each isolate and the dose of metacercariae given to each sheep is shown. Patency of infection was confirmed by faecal egg count and one sheep from each pair was treated with 10 mg/kg triclabendazole (T) 10 days prior to post-mortem (PM) with the exception of FhLivR3 where one sheep had to be euthanized during the course of the experiment. The number of adult flukes recovered from each sheep at PM is shown
^bActual number not calculated
Abbreviations: wpi weeks post infection, PM post-mortem

ISSN: 1756-3305