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Table 2 Experimental infection of sheep for production of Fasciola hepatica clonal isolates

From: Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones

Clonal isolate Total no. of cercariae shed by a single snail used to derive clonal infections No. of metacercariae used for infection Timepoint of PM (wpi) No. of adult flukes recovered at PMa
FhLivR1 931 150 18 70
220 18 165 (T)
FhLivR2 553 193 18 31
200 18 48 (T)
FhLivR3 812 256 18 35 (T)
FhLivS1 3200 209 19 15
224 19 0 (T)
FhLivS2 > 417b 215 24 113
202 24 0 (T)
FhLivS3 1166 200 17 127
200 17 0 (T)
  1. aTwo sheep were infected for each isolate and the dose of metacercariae given to each sheep is shown. Patency of infection was confirmed by faecal egg count and one sheep from each pair was treated with 10 mg/kg triclabendazole (T) 10 days prior to post-mortem (PM) with the exception of FhLivR3 where one sheep had to be euthanized during the course of the experiment. The number of adult flukes recovered from each sheep at PM is shown
  2. bActual number not calculated
  3. Abbreviations: wpi weeks post infection, PM post-mortem