Fig. 1

A Volcano plot showing differentially expressed genes of T. congolense parasites in the cardia relative to those in the probosces of G. morsitans. Only transcripts with at least 30 reads mapped and at least five RPKM (Reads Per Kilobase Million) by CLC-Genomics and EdgeR analysis [36, 99] in either library (C-parasites or PB-parasites) were considered. Red dots indicate DE genes with an FC of ≥ 2 (log₂ = 1) and false detection rate (FDR) corrected P-value of < 0.05 between cardia and proboscis parasites. The x-axis displays magnitude of fold-changes and y-axis the statistical significance (-log₁₀ of P-value). Points having FC of < 2 (log₂ < 1) on an FDR corrected P-value of < 0.05 are shown in black, and indicate genes with non-significance change between different developmental states