Fig. 2

The total number and maturation phenotype of CD11c⁺CXCR5⁺ DCs were comparable between immunized WT and PD-1^-/- mice. a-c Splenocytes were isolated from the immunized WT (n = 5) and PD-1^-/- mice (n = 5) at 2, 4 and 6 days after the initial immunization. a Statistical analysis of the total number of CD11c⁺CXCR5⁺ DCs in the spleen from ITV-immunized WT and PD-1^-/- mice on days 2, 4 and 6 after the initial immunization. b Gating strategy and FACS analysis of the expression of CD40, CD86, and MHC-II on CD11c⁺CXCR5⁺ DCs from immunized WT (thin line) and PD-1^-/- (bold line) mice. Spleen lymphocytes were gated as CD11c⁺CXCR5⁺, and the expression levels of CD40, CD86 and MHC-II were analyzed. c A graphical representation of the geometrical mean of the immunofluorescence intensity of the expression of all three markers. Three independent experiments were performed. The data are presented as the mean ± SD. Data were compared with two-way ANOVA. Abbreviation: n, number of samples