Hints on T cell responses in a fish-parasite model: Enteromyxum leei induces differential expression of T cell signature molecules depending on the organ and the infection status

Table 4 Enteromyxum leei induces a shift towards a T_H1 environment in the intestine of gilthead sea bream

Organ	Head kidney		Spleen		Anterior intestine		Posterior intestine
Group	CTRL	RCPT	CTRL	RCPT	CTRL	RCPT	CTRL	RCPT
tbet / gata3 ratio	3.90 ± 0.43^a	5.10 ± 0.73	2.54 ± 0.40^a	2.65 ± 0.23	0.53 ± 0.09^b	1.45 ± 0.13*	0.94 ± 0.14^b	1.13 ± 0.10
ifnγ / il4 / 13a ratio	6.72 ± 3.08^a	4.16 ± 1.62	18.35 ± 5.14^a	9.79 ± 2.45	1.06 ± 0.33^b	2.63 ± 0.52*	0.59 ± 0.10 ^b	1.74 ± 0.38*

The T_H1 / T_H2 balance was assessed as the ratio of tbet / gata3 and ifnγ / il4 / 13a expression values for each individual fish in the study. Data are shown as mean ± SEM for n = 10 fish. Asterisks (*) indicate significant differences between E. leei-exposed (RCPT) and control (CTRL) groups within each organ (t-test, P < 0.05). Different superscript letters indicate significant differences in the CTRL groups among all organs within each calculated ratio (ANOVA, P < 0.05)

ISSN: 1756-3305