Table 1 Primer sets of B. bigemina homologs of P. falciparum gamete-specific genes
Gene | Forward primer (5'-3') | Reverse primer (5'-3') | Amplicon size (bp) |
|---|---|---|---|
BBBOND_0204030 | GAGGAAATCGATGGGACGTATG | GGTACTCCATGTGACGTTTAGG | 496 |
BBBOND_0204030 (nested PCR) | ACATCTCTGCGTCTTGCATAA | GGACTGTATGTATGCCCTTCAG | 204 |
BBBOND_0310420 | GAGCAGGTGGCGACATATT | CCTTGATACCGTTTCCCATAGAG | 330 |
BBBOND_0110620 | GTCGAGAAAGGCCAGGATAAA | TGTGTCATGGGAGGGTTTG | 382 |
BBBOND_0308930 | CGAGGGCGCTACTCTATTC | CACTGATGTTTCGTGGCTTAC | 267 |
BBBOND_0200260 | ATGTCGAAGGATTCCACTGC | CTTGCGAGAGGATGGTGTG | 208 |
BBBOND_0311670 | GAAGCCTGAGTGGAACCTAAA | GCGGAGGTGGGATTCATATT | 343 |
BBBOND_0208380 | TCGTCGCGTGGAATTCATAG | TGTTCACCGCCTCGAAATAG | 449 |
BBBOND_0305250 | GCGAGTACTGCGGCATTTA | CTGAAGCCAAGCTGAGGTG | 295 |
BBBOND_0307580 | CGAAGATCCTCGTGCTGATAAT | GAGCGGGTTGTATAGGTACTTG | 386 |
BBBOND_0310760 | TGCTTCACCTGCGGCAA | TCAGATGTGCTCCTGGTTCC | 204 |
BBBOND_0311270 | ATCTGCAAGTTCTGGGAGAAG | CATGTAGCCCTGCTGTTGATA | 477 |
BBBOND_0203950 (CCp2) | TCCACAACAAGCAACTCC | GCTTTGTAGGTAGAGTCAGC | 168 |
BBBOND_0312400 (CCp3) | GAGTCCTCCGTGTAGATGAAC | GAAGCTTACTTGCTACGACAAC | 141 |
BBBOND_0307410 (HAP2) | GCTGAAGACGAAGGTGAGGA | GCGATGATCTGGGTGACG | 164 |
BBBOND_0107357 (actin) | ATCGCCGTTTACACTTCACG | GCCCCTTCCTCCTCGTAATC | 131 |