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Table 2 Treatment conditions to induce B. bigemina sexual stages. All cultures were in the presence of 5% CO2. Bold indicates sexual stage induction

From: Identification of a putative methyltransferase gene of Babesia bigemina as a novel molecular biomarker uniquely expressed in parasite tick stages

Condition Inducer Concentrations (mM)
26 °C TCEP 0 10 20 25 30 40
TCEP+ XA 0 10 20 25 30 40
37 °C TCEP 0 10 20 25 30 40
TCEP+ XA 0 10 20 25 30 40
26 °C DTT 0 10 20 25 30 40
DTT+ XA 0 10 20 25 30 40
37 °C DTT 0 10 20 25 30 40
DTT+ XA 0 10 20 25 30 40
  1. Abbreviations: TCEP, Tris 2-carboxyethyl phosphine; DTT, dithiothreitol; XA, xanthurenic acid