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Fig. 4 | Parasites & Vectors

Fig. 4

From: Characterization of a serine protease inhibitor from Trichinella spiralis and its participation in larval invasion of host’s intestinal epithelial cells

Fig. 4

Far Western analysis of rTsSPI binding to IEC proteins. The IEC proteins were analyzed by SDS-PAGE, subsequently the IEC protein binding with rTsSPI was detected in a Far Western analysis. a SDS-PAGE analysis of IEC proteins. Lane M: protein marker; Lane 1: IEC lysates; Lane 2: C2C12 lysates. b Far-Western analysis of IEC protein binding to rTsSPI. The IEC protein was first incubated using rTsSPI (Lanes 1–3), IL1 ES proteins (Lanes 4–6) or BSA (Lanes 7–9), subsequently recognized by anti-rTsSPI serum (Lanes 1, 4 and 7), infection serum (Lanes 2, 5 and 8), and pre-immune normal serum (Lanes 3, 6 and 9). c Far Western analysis of C2C12 protein binding to rTsSPI. The C2C12 protein (Lanes 1–3) was first incubated with rTsSPI, and subsequently incubated with anti-rTsSPI serum (Lane 1), infection serum (Lane 2) or pre-immune serum (Lane 3). There was no binding between rTsSPI and the C2C12 protein

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Parasites & Vectors

ISSN: 1756-3305

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