Table 1 Primer sets used for the synthesis of dsRNA. The positions of the primers with respect to SDNP sequence (GenBank: GU222920), sequence corresponding to T7 promoter region of primers (underlined), the size of the PCR products, and the optimized annealing temperatures of primer sets are given
Primer | Position | Sequence (5'-3') | Product size (bp) | Annealing T (°C) |
|---|---|---|---|---|
SDNP-1-F | 1 | TAATACGACTCACTATAGGGCGACGAGGGTTCCATTGAGTGA | 154 | 55 |
SDNP-1-R | 134 | TAATACGACTCACTATAGGGGCTTCCTGACAAGCCAAACAT | ||
SDNP-2-F | 155 | TAATACGACTCACTATAGGGCGACGAGGGTTCCATTGAGTGA | 150 | 55 |
SDNP-2-R | 282 | TAATACGACTCACTATAGGGTTGGAATATAAACATGCGGTATA | ||
SDNP-3-F | 305 | TAATACGACTCACTATAGGGCTACCGGTATCAGAACTCAGAA | 157 | 55 |
SDNP-3-R | 462 | TAATACGACTCACTATAGGGTAGCGCTGGACCGAATTCTTTT | ||
SDNP-4-F | 463 | TAATACGACTCACTATAGGGGGAAATGGAACTTGTGAAATAA | 155 | 45 |
SDNP-4-R | 618 | TAATACGACTCACTATAGGGTCAGTAATTAATCAAATT |