Fig. 3

Functional complementation of a temperature-sensitive α-SNAP mutant of yeast with the putative SNAPs of Giardia. Temperature-sensitive sec17-1mutant yeast strain (RSY269) was transformed with constructs expressing either SEC17 (positive control) or each of the giardial SNAPs; transformants carrying the empty vector served as negative control. Transformants were spotted onto synthetic medium lacking uracil and incubated at either 30 °C (permissive temperature) or 37 °C (non-permissive temperature)