Table 2 In vitro effects of esculin on trehalase activity of adult female Lutzomyia longipalpis. Tissue homogenates were incubated or not with esculin, and enzyme assays were performed using trehalose as a substrate. Tissues were homogenized and separated by centrifugation into gut tissue, gut contents, rest of body tissues and rest of body soluble fraction. Results are presented as μU/insect and are the mean and SEM of 8 biological replicates with 4 insects in each sample
Sample | Control (trehalose only) | Esculin added to the assay mixture |
|---|---|---|
Gut, tissue | 380 ± 70 | 300 ± 100 |
Gut, contents | 170 ± 60 | 220 ± 90 |
Rest of body, tissues | 560 ± 60a | 380 ± 50a |
Rest of body, soluble fraction | 580 ± 10b | 360 ± 20b |