Table 5 Effect of supplementing the sucrose diet with esculin on β-glucosidase activities in female Lutzomyia longipalpis. Tissue homogenates were assayed using cellobiose as a substrate. Tissues were homogenized and separated by centrifugation into gut tissue, gut contents, rest of body tissues and rest of body soluble fraction. Results are presented as μU/insect and are the mean and SEM of 5 biological replicates with 10 insects in each sample
Sample | Control (sucrose only) | Esculin supplemented |
|---|---|---|
Gut, tissue | 1.3 ± 0.8 | 2.2 ± 0.8 |
Gut, contents | 8 ± 3 | 2 ± 1 |
Rest of body, tissues | 1.6 ± 1.0 | 3 ± 1 |
Rest of body, soluble | 0a | 8 ± 1a |