Fig. 4
From: Editing the genome of Aphanomyces invadans using CRISPR/Cas9
Coding sequence (mRNA sequence) of Aphanomyces invadans’ hypothetical protein (GenBank: XM_008877667) contains 1388 nucleotides. The designed gRNA2 site is highlighted in green and the PAM site in grey. Forward and reverse primer 2 (product size 214 bp) sites are highlighted in yellow. The point mutation (deletion) located at the 5th nt from the PAM site is shown in red within the gRNA2 sequence