Table 1 Demographic, clinical and parasitological data, Blastocystis subtypes by 18S rDNA and clades by PFOR
Sample | Gendera/ageb | Symptoms | Parasite load (CPS-40×) | Stage-morphotype culture (48 h) | Subtypes (18S rDNA) | Clades (PFOR) | |
|---|---|---|---|---|---|---|---|
Santin primers set | subtype-specific primers set | ||||||
01 | M/4 | Abdominal pain | > 5 | Vacuolar | ST1 (MH453913) | ST1 | I (MH507339) |
02 | F/11 | Abdominal pain | > 5 | Vacuolar | ST3 (MH453914) | ST3 | I (MH507340) |
03 | F/13 | Abdominal pain and diarrhoea | > 10 | Vacuolar, granular, amoeboid | ST3 (MH453915) | ST3 | III (MH507341) |
04 | M/7 | Abdominal pain | > 5 | Vacuolar | ST2 (MH453916) | ST2 | II (MH507342) |
06 | M/14 | Abdominal pain | > 5 | Vacuolar | ST1 (MH453917) | ST1, ST2 | II (MH507343) |
07 | F/10 | Abdominal pain | > 5 | Vacuolar | ST2 (MH453918) | ST2 | II (MH507344) |
08 | F/9 | Abdominal pain | > 5 | Vacuolar | ST3 (MH453919) | ST3 | III (MH507345) |
09 | F/7 | Abdominal pain | > 5 | Vacuolar | ST3 (MH453920) | ST3 | I (MH507346) |
11 | M/13 | Abdominal pain | > 5 | Vacuolar | ST3 (MH453921) | ST3 | III (MH507347) |
14 | M/3 | Abdominal pain and diarrhoea | > 10 | Vacuolar, granular, amoeboid | ST3 (MH453922) | ST3 | I (MH507348) |
15 | M/7 | Abdominal pain and mucous stool | > 10 | Vacuolar, granular | ST3 (MH453923) | ST3 | III (MH507349) |
21 | F/1 | Abdominal pain | > 5 | Vacuolar | ST1 (MH453924) | ST1 | I (MH507350) |
22 | M/14 | Abdominal pain | > 10 | Vacuolar, granular | ST1 (MH453925) | ST1 | I (MH507351) |
24 | M/6 | Abdominal pain | > 5 | Vacuolar | ST7 (MH453926) | NDc | I (MH507352) |
25 | F/10 | Abdominal pain | > 5 | Vacuolar | ST1 (MH453927) | ST1, ST2 | I (MH507353) |
33 | M/8 | Abdominal pain and diarrhoea | > 5 | Vacuolar | ST3 (MH453928) | ST3 | III (MH507354) |
35 | F/8 | Abdominal pain and diarrhoea | > 5 | Vacuolar | ST1 (MH453929) | ST1 | I (MH507355) |
102 | F/8 | Abdominal pain and diarrhoea | > 10 | Vacuolar | ST3 (MH453930) | ST3 | III (MH507356) |
37 | F/9 | Abdominal pain | > 10 | Vacuolar | ST2 (MH453931) | ST2 | II (MH507357) |
45 | M/3 | Abdominal pain | > 5 | Vacuolar | ST1 (MH453932) | ST1 | I (MH507358) |
46 | M/5 | Abdominal pain and diarrhoea | > 5 | Vacuolar | ST1 (MH453933) | ST1 | I (MH507359) |
ATCC-50754 | – | – | – | – | ST3 (MH453934) | – | III (MH507360) |