West Nile virus lineage 2 in Romania, 2015–2016: co-circulation and strain replacement

Parasites & Vectors

Table 1 West Nile virus lineage 2 strains circulating in Romania, 2015–2016

Study year	Samples	Location	Date of sampling	WNV strain based on NS5 sequence	No. of isolates sequenced	Position 159 in E glycoprotein	Position 249 in NS3 protein
2015	Mosquitoes	Tulcea county site	6-29 August	Eastern European clade	3	M	H
		Tulcea county site	Aug-28	Central/Southern European clade^a	1	T	P
		Bucharest city	Jul 9-Aug 19	Eastern European clade	3	na	H
		Bucharest city	Aug-20	Central/Southern European clade^a	1	na	P
	Human	Sibiu	September 11 (onset)	Central/Southern European clade	1	T	P
2016	Mosquitoes	Tulcea county site	Aug 5-Sep 22	Central/Southern European clade^a	8	T	P
	Mosquitoes	Bucharest city	Jun-21	Central/Southern European clade^a	1	na	P
	Human	Bucharest city	Aug-31	Central/Southern European clade^a	1	T	P

Abbreviations: H histidine, M methionine, P proline, T threonine, na not assessed
^aSoutheastern Romania cluster

ISSN: 1756-3305