Fig. 2

Localization of F. gigantica-derived rFgRab10 protein to the PBMCs surface. rFgRab10 protein binding to goat PBMCs surface was determined by incubating PBMCs treated with purified rFgRab10 protein, protein from sonicated culture of E. coli transformed with empty pET-SUMO vector or sham-treated with PBS (control) with primary anti-rFgRab-10 protein antibody raised in rats. PBMCs’ nuclei and rFgRab10 protein were stained by Hoechst 33342 (blue) and Cy3-conjugated secondary antibody (red), respectively. Surface staining was detected in rFgRab10-treated cells, but not in PBMCs incubated with SUMO protein and sham-treated PBMCs. Scale-bars: 10 μm