Fig. 4

Inhibition of OATPs affect LGTV loads in tick cells. a Representative phase-contrast images of LGTV-infected mock-treated or SPZ-treated tick cells (4 h post-treatment, before infection) followed by 24 h post-LGTV-infection. b QRT-PCR analysis showing levels of viral RNA in LGTV-infected mock- or SPZ-treated tick cells at 24 h pi. Open circles represent data from LGTV-infected mock-treated and closed circles represent data from SPZ-treated tick cells at 24 h pi. Each circle represents data from one cell culture well. LGTV loads were normalized to the amount of tick beta-actin levels. In b, the P-value indicates the result from statistical analysis. Scale-bars: 200 μm