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Table 1 Babesia bovis ron2 designed primers

From: Babesia bovis RON2 contains conserved B-cell epitopes that induce an invasion-blocking humoral immune response in immunized cattle

Primer

Sequence (5'-3')

Fw0 RON2Bo

CACCTCACCGATATGCGTAC

RvI0 RON2Bo

GAGTCACTGACACCTTGCC

FwI0 RON2Bo

GTCAGTGACTCCCCTCTTCAAG

Rv0 RON2Bo

GGAATCACCGCCTAGTAGC

Fw1 RON2Bo

GCTACTAGGCGGTGATTCC

RvI1 RON2Bo

GCGTCAATAGAGATAAGCAGG

FwI1 RON2Bo

CCTGCTTATCTCTATTGACGC

Rv1 RON2Bo

CAACCCATTGCTTGATTCCC

Fw2 RON2Bo

GGGAATCAAGCAATGGGTTG

RvI2 RON2Bo

CTTTCTTAGCAATAGCGTCGG

FwI2 RON2Bo

CTTCGTTGCTGGAGGCTACATC

Rv2 RON2Bo

CGTTGGATATTCGGTTGAGC

Fw3 RON2Bo

GCTCAACCGAATATCCAACG

Rv3 RON2Bo

CCGTACTTGATTGCTCTGAG

Fw4 RON2Bo

CTCAGAGCAATCAAGTACGG

Rv4 RON2Bo

CACGGATGGCTATGACAATG

  1. Note: Eight pairs of primers were designed to get the amplification and sequencing of 4169 bp under the same PCR protocol
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Parasites & Vectors

ISSN: 1756-3305

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