|
Primer
|
Sequence (5'-3')
|
|---|
|
Fw0 RON2Bo
|
CACCTCACCGATATGCGTAC
|
|
RvI0 RON2Bo
|
GAGTCACTGACACCTTGCC
|
|
FwI0 RON2Bo
|
GTCAGTGACTCCCCTCTTCAAG
|
|
Rv0 RON2Bo
|
GGAATCACCGCCTAGTAGC
|
|
Fw1 RON2Bo
|
GCTACTAGGCGGTGATTCC
|
|
RvI1 RON2Bo
|
GCGTCAATAGAGATAAGCAGG
|
|
FwI1 RON2Bo
|
CCTGCTTATCTCTATTGACGC
|
|
Rv1 RON2Bo
|
CAACCCATTGCTTGATTCCC
|
|
Fw2 RON2Bo
|
GGGAATCAAGCAATGGGTTG
|
|
RvI2 RON2Bo
|
CTTTCTTAGCAATAGCGTCGG
|
|
FwI2 RON2Bo
|
CTTCGTTGCTGGAGGCTACATC
|
|
Rv2 RON2Bo
|
CGTTGGATATTCGGTTGAGC
|
|
Fw3 RON2Bo
|
GCTCAACCGAATATCCAACG
|
|
Rv3 RON2Bo
|
CCGTACTTGATTGCTCTGAG
|
|
Fw4 RON2Bo
|
CTCAGAGCAATCAAGTACGG
|
|
Rv4 RON2Bo
|
CACGGATGGCTATGACAATG
|
- Note: Eight pairs of primers were designed to get the amplification and sequencing of 4169 bp under the same PCR protocol
