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Table 3 Results of LC-MS/MS analysis of Trichinella britovi muscle larvae (ML) selected spots which reacted with pig sera collected at 10 dpi

From: Trichinella britovi muscle larvae and adult worms: stage-specific and common antigens detected by two-dimensional gel electrophoresis-based immunoblotting

Spot

NCBIprot accession No.

MSa

MPb

Seqc

SC (%)d

emPAIe

Mr(kDa)/pIf

Description

24

a

KRY11608.1

1765

36

26

36

4.01

75.526/6.24

Intermediate filament protein B

KRY09282.1

1457

30

22

30

2.92

73.168/6.07

Intermediate filament protein ifa-1

b

KRY11608.1

1903

40

29

39

5.11

75.526/6.24

Intermediate filament protein B

KRY09282.1

1541

33

23

33

3.30

73.168/6.07

Intermediate filament protein ifa-1

c

KRY11608.1

930

14

13

19

1.24

75.526/6.24

Intermediate filament protein B

KRY09282.1

2328

66

34

47

7.99

73.168/6.07

Intermediate filament protein ifa-1

29

 

XP_003373575.1

1206

52

15

41

4.74

42.210/5.30

Actin-5C

30

 

XP_003373575.1

527

9

8

25

1.47

42.210/5.30

Actin-5C

 

KRY50178.1

415

8

6

15

0.89

46.783/5.44

Hypothetical protein T03_17187

 

KRZ06996.1

160

3

3

4

0.12

74.844/5.78

Intermediate filament protein ifa-1, partial

 

KRZ09733.1

323

5

5

5

0.28

96.031/6.00

Mitochondrial-processing peptidase subunit beta, partial

 

KRX47705.1

293

4

4

3

0.14

150.442/6.28

Serine protease 30

31

 

KRZ02603.1

1083

28

14

34

2.98

50.922/6.01

Enolase, partial

 

KRY13126.1

544

10

9

20

1.47

48.623/5.41

26S protease regulatory subunit 7

32

 

KRY18793.1

883

19

14

30

3.45

54.997/5.00

Protein disulfide-isomerase 2

 

OUC40875.1

749

17

10

26

2.73

48.387/4.87

Putative Tubulin/FtsZ family, GTPase domain protein

 

KRY00151.1

655

14

8

17

1.31

59.844/5.54

ATP synthase subunit beta, mitochondrial

33

 

KRX41020.1

1127

27

16

27

1.92

72.856/5.09

Heat-shock 70 kDa protein C, partial

 

KRY00702.1

364

6

5

9

0.46

68.894/5.08

V-type proton ATPase catalytic subunit A

34

 

Unidentified

–

–

–

–

–

–

–

  1. aMascot score
  2. bMatched peptide
  3. cSequence
  4. dSequence coverage (%)
  5. eExponentially modified protein abundance index
  6. fExperimental nominal mass (kDa) and isoelectric point