Table 1 Identities associated with differentially expressed proteins in the comparison between TcI and TcII genotypes
Spot no./Strain | Accession number | Description | Gene | Unique peptides | Coverage (%) | pI | Observed MW (kDa) |
|---|---|---|---|---|---|---|---|
Translation/Transcription | |||||||
 22/PR150 | TCSYLVIO_990036 | Elongation factor 2 | EF2 | 31 | 42.25 | 6.04 | 75.70 |
 101/PR150 | TcCLB.503419.50 | RNA-binding protein RGGm | RGG2 | 8 | 27.51 | 8.76 | 34.45 |
 139/PR150 | TcCLB.506925.130 | Eukaryotic translation initiation factor 5a | EIF5A | 7 | 58.68 | 5.00 | 9.88 |
 56/452 | TcCLB.509583.10 | Chaperonin TCP20 | TP20 | 22 | 41.07 | 7.69 | 51.11 |
 72/452 | TCSYLVIO_002198 | Elongation factor 1-alpha (EF-1-alpha) | EF1A | 1 | 44.79 | 8.98 | 43.99 |
Heat-shock response | |||||||
 28/PR150 | TCSYLVIO_008621 | Heat shock protein 85 | HSP85 | 6 | 10.99 | 6.35 | 73.76 |
 43/PR150 | TCSYLVIO_004969 | Chaperonin HSP60, mitochondrial precursor | HSP60 | 44 | 66.55 | 5.50 | 65.35 |
 44/PR150 | TCSYLVIO_004969 | Chaperonin HSP60, mithocondrial precursor | HSP60 | 36 | 75.09 | 5.50 | 65.35 |
 71/PR150 | TCSYLVIO_004831 | Thiol-dependent reductase 1 | TDR1 | 11 | 36.63 | 5.97 | 46.04 |
 26/452 | TcCLB.507713.30 | Heat-shock protein 85 | HSP85 | 33 | 43.18 | 5.15 | 74.41 |
 35/452 | TcCLB.511211.170 | Heat-shock protein 70 (HSP70) | HSP70 | 6 | 48.01 | 5.57 | 69.23 |
 41/452 | TCSYLVIO_003281 | Heat-shock 70 kDa protein, mithocondrial precursor | HSP70 | 33 | 46.56 | 6.13 | 67.29 |
 42/452 | TCSYLVIO_003281 | Heat-shock 70 kDa protein, mithocondrial precursor | HSP70 | 15 | 27.94 | 6.13 | 66.00 |
 125/452 | TcCLB.506207.50 | IgE-dependent histamine-releasing factor | HRF | 4 | 16.47 | 4.64 | 18.35 |
 131/452 | TcCLB.506207.50 | IgE-dependent histamine-releasing factor | HRF | 6 | 21.18 | 4.64 | 16.00 |
 131/452 | TcCLB.509775.40 | Iron superoxide dismutase | SODB | 3 | 56.65 | 8.60 | 16.00 |
 140/452 | TcCLB.503.899.130 | Glutathione peroxidase-like protein | GPX | 8 | 39.33 | 6.00 | 8.47 |
Cell signalling | |||||||
 105/452 | TcCLB.503855.20 | Spermidine synthase | SPSYN | 1 | 35.14 | 5.41 | 27.61 |
 140/452 | TcCLB.503715.30 | Ras-related protein RAB1A | RAB1A | 3 | 15.71 | 7.77 | 8.47 |
 143/452 | TcCLB.511407.60 | Small GTP-binding protein RAB11 | RAB11 | 7 | 43.78 | 8.13 | 3.36 |
Protein synthesis/Degradation | |||||||
 59/PR150 | TCSYLVIO_005562 | Hypothetical protein (Metalocarboxypeptidase1) | MCP1 | 19 | 50.21 | 5.86 | 48.66 |
 108/PR150 | TCSYLVIO_001046 | Peptidase M20/M25/M40 | MT2598 | 8 | 17.78 | 5.43 | 27.61 |
 137/PR150 | TcCLB.507639.40 | Proteasome beta 5 subunit | PSMB5 | 9 | 27.01 | 5.90 | 13.47 |
 127/452 | TCSYLVIO_005774 | Proteasome beta 2 subunit | PSMB2 | 9 | 24.66 | 9.19 | 18.35 |
Carbohydrate metabolism | |||||||
 24/PR150 | TCSYLVIO_008551 | Pyruvate phosphate dikinase | PPDK | 3 | 37.18 | 6.73 | 75.05 |
 99/PR150 | TCSYLVIO_002700 | Cytosolic malate dehydrogenase | MDH | 9 | 35.24 | 6.73 | 34.45 |
 42/452 | TCSYLVIO_004608 | Phosphoglycerate mutase | PGMI | 26 | 61.01 | 5.92 | 66.00 |
 51/452 | TcCLB.507547.90 | Glycosomal phosphoenolpyruvate carboxykinase | PEPCK | 18 | 38.86 | 8.37 | 62.76 |
 106/452 | TcCLB.510105.230 | Glyceraldehyde 3-phosphate dehydrogenase | GAPDH | 19 | 55.92 | 8.46 | 28.07 |
Nucleotide metabolism | |||||||
 34/PR150 | TCSYLVIO_003291 | Tetrahydrofolate synthase | MTHFD1 | 10 | 16.67 | 7.68 | 69.23 |
 46/452 | TcCLB.508731.60 | Adenylosuccinate synthetase | ADSS | 7 | 45.13 | 8.21 | 63.46 |
Aminoacid metabolism | |||||||
 34/PR150 | TCSYLVIO_006380 | Urocanate hydratase | UROC1 | 10 | 14.67 | 6.86 | 69.23 |
 36/PR150 | TCSYLVIO_006380 | Urocanate hydratase | UROC1 | 16 | 20.74 | 6.86 | 69.23 |
 38/PR150 | TCSYLVIO_006380 | Urocanate hydratase | UROC1 | 37 | 44.74 | 6.86 | 68.58 |
 80/PR150 | TCSYLVIO_000769 | Tyrosine aminotransferase | TAT | 7 | 49.05 | 6.54 | 42.38 |
 56/452 | TCSYLVIO_004599 | Histidine ammonia-lyase | HAL | 16 | 29.59 | 8.34 | 51.11 |
 93/452 | TCSYLVIO_001538 | Aspartate aminotransferase | GOT1 | 13 | 42.34 | 8.81 | 36.50 |
 111/452 | TCSYLVIO_006042 | Arginase | ARG | 12 | 54.22 | 6.46 | 25.88 |
 112/452 | TCSYLVIO_003182 | Pyridoxal kinase | PDXK | 1 | 23.00 | 6.44 | 20.23 |
Lipid metabolism | |||||||
 113/452 | TcCLB.506213.50 | Prostaglandin F synthase | PGFS | 4 | 15.66 | 8.82 | 24.00 |
 115/452 | TcCLB.511693.90 | Electron-transfer-flavoprotein, alpha polypeptide | ETFA | 4 | 57.01 | 8.27 | 24.00 |
 116/452 | TcCLB.506213.50 | Prostaglandin F synthase | PGFS | 8 | 30.25 | 8.82 | 24.00 |
 118/452 | TcCLB.510105.240 | Short chain 3-hydroxyacyl-coa dehydrogenase | HCD2 | 9 | 34.69 | 8.63 | 23.50 |
Cytoskeleton | |||||||
 28/PR150 | TCSYLVIO_007343 | Calpain-like cysteine peptidase | SMP-1 | 10 | 15.03 | 5.05 | 73.76 |
 102/452 | TcCLB.506563.40 | Beta tubulin | TUBB | 9 | 33.26 | 4.81 | 29.92 |
 142/452 | TCSYLVIO_008641 | Epsilon tubulin | TUBE1 | 6 | 15.58 | 6.52 | 7.05 |