Fig. 1
Phylogenetic relationships between SsCLPs and CLPs and chitinases from other species. The unrooted phylogenetic tree was constructed using CLP and chitinase sequences from approximately 40 species by the maximum likelihood (ML) method in MEGA software. CLP sequences or chitinase sequences were used in the tree (with their GenBank or SwissProt accession numbers). The number behind the protein acronym (for example Chi1, CLP2, CLP3, etc.) is from the NCBI database which represents more than one kind of protein in the species. Accession numbers are as follows: Stegodyphus mimosarum-Chi2 (gb: KFM81240.1); Parasteatoda tepidariorum-CLP1 (gb: XP_015906877.1); Limulus polyphemus-CLP1 (gb: XP_013781598.1); S. scabiei-CLP11 (gb: KPM07813.1); Tetranychus cinnabarinus-Chi1 (gb: AQX83302.1); Tetranychus urticae-Chi1 (gb: XP_015789865.1); S. scabiei-CLP8 (gb: KPM09395.1); E. maynei-CLP (gb: OTF83884.1); Nilaparvata lugens-Chi2 (gb: XP_022199541.1); Bactrocera dorsalis-Chi (gb: AEN03034.1); S. scabiei-CLP4 (gb: KPM08718.1); B. dorsalis-Chi8 (gb: AQR60114.1); Drosophila willistoni-CLP1 (gb: XP_002074824.1); Copidosoma floridanum-CLP1 (gb: XP_014210861.1); Aedes aegypti-CLP (gb: EAT45692.1); Melipona quadrifasciata-Chi1 (gb: KOX77106.1); Bombus terrestris-Chi1 (gb: XP_003395393.1); S. scabiei-CLP6 (gb: KPM08912.1); Homarus americanus-Chi (gb: ABQ59095.1); T. cinnabarinus-Chi (gb: APA05224.1); T. urticae-Chi3 (gb: XP_015795759.1); S. scabiei-CLP12 (gb: KPM07848.1); Haemaphysalis longicornis-Chi (gb: BAC06447.1); Anopheles sinensis-CLP (gb: KFB36184.1); N. lugens-Chi (gb: AJO25042.1); Papilio xuthus-Chi3 (gb: KPJ02577.1); Bombyx mori-Chi10 (gb: XP_021203926.1); Drosophila grimshawi-Chi (gb: XP_001991892.1); Drosophila melanogaster-Chi (gb: NP_001245599.1); Zootermopsis nevadensis-CLP1 (gb: XP_021912899.1); T. cinnabarinus-CLP1 (gb: ARN16744.1); S. scabiei-CLP1 (gb: KPM05982.1); Nephila clavipes-CLP1 (gb: PRD36178.1); Ixodes scapularis-Chi (gb: XP_002403819.1); S. scabiei-CLP2 (gb: KPM07295.1); E. maynei-CLP (gb: OTF69711.1) ; Spodoptera litura-CLP1 (gb: XP_022814601.1); S. scabiei-CLP9 (gb: KPM10408.1); E. maynei-CLP (gb:OTF70345.1); I. scapularis-Chi (gb: XP_002434337.1); Cimex lectularius-CLP (gb: XP_014241842.1); P. tepidariorum-CLP (gb: XP_015916511.1); S. mimosarum-Chi (gb: KFM61851.1); S. scabiei-CLP3 (gb: KPM07498.1 ); E. maynei-CLP (gb: OTF83991.1); S. scabiei-CLP10 (gb: KPM11497.1); E. maynei-CLP (gb: OTF77275.1); T. urticae-CLP (gb: XP_015790837.1); P. xuthus-Chi1 (gb: KPI93103.1); N. lugens-Chi (gb: AJO25038.1); Harpegnathos saltator-Chi (gb: EFN85614.1); Camponotus floridanus-CLP (gb: EFN63520.1); Struthio camelus australis-CDCP1 (gb: KFV81001.1 ) CDCP1; Egretta garzetta-CDCP1 (gb: KFP09506.1); Oryzias melastigma-CDCP (gb: XP_024137025.1); S. scabiei-CLP5 (gb: KPM08736.1); E. maynei-CLP (gb: OTF80145.1); L. polyphemus-CDCP (gb: XP_013787202.1); Daphnia magna-CDCP (gb: KZS04185.1); S. scabiei-CLP7 (gb: KPM08913.1) and E. maynei-CLP (gb: OTF84112.1). Abbreviations: gb, GenBank or SwissProt accession numbers; Chi, chitinase; CLP, chitinase-like protein; CDCP, chitinase domain-containing protein